Journal: Science Advances
Article Title: Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
doi: 10.1126/sciadv.abp9435
Figure Lengend Snippet: ( A ) Schematic illustration of exosome for in vivo delivery of Cas9 RNP for the treatment of CCL 4 -induced liver fibrosis. CCL 4 was administrated through intraperitoneal injection, and exosome RNP complexes were administered through tail vein. ( B ) Frequency of indel mutation detected by T7E1 assay from liver tissues after the specified treatments at day 35. ( C ) Frequency of indel mutation detected by T7E1 assay from different organs after exosome RNP treatment at day 35. ( D ) Western blotting of CcnE1 and α-SMA in livers of mice after the specified treatments. ( E ) H&E staining of the liver sections from the mice after the specified treatments. Scale bar, 200 μm. ( F to H ) Assessment of fibrosis progression by semiquantitative scoring (Ishak) (F), determination of morphometric collagen1α1 (G), and hydroxyproline content (H). Means ± SD; n = 6 (one-way ANOVA with a Tukey’s post hoc test, *** P < 0.001).
Article Snippet: Collagen1α1 expression in whole-liver extracts was determined by the Mouse Collagen1α1 ELISA Kit (Sangon Biotech).
Techniques: In Vivo, Injection, Mutagenesis, Western Blot, Staining